Extraction of Sugars from Black Gram Peels by Reversed-Phase Liquid Chromatography Systems and Identification by TLC and Mass Analysis

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Identification and Analysis of the Migration of Primary Aromatic Amines from Flexible Food Packaging by High-performance Liquid Chromatography (HPLC)

Background and Objectives: Primary Aromatic Amines (PAAs) are contaminants that can migrate from food flexible packaging. The main origin of these compounds is polyurethane adhesives used in the laminating process of multilayer films. Residual aromatic di-isocyanates of the reaction of adhesive polymerization migrate into the food, and are hydrolyzed to PAAs by the moisture of food. 2,4- and 2,...

Extraction and Detection of Pentobarbital in Soil by Solid Phase Extraction and Liquid Chromatography / Mass Spectrometry

Chemically Modified Eggshell Membrane as an Adsorbent for Solid-Phase-Extraction of Morphine Followed by High Performance Liquid Chromatography Analysis

In this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by HPLC analysis. At first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (<70%), modification of eggshell membrane surface was carried out using different surfactants and amines. The amine modified eggshells characte...